![]() Sophisticated pulse sequence elements reduce DQCOSY-like +/- zero-quantum coherence (ZQC) artifacts. Gives good signal on 10 mg samples.ĢD NOESY (Nuclear Overhauser Effect SpectroscopY) for detecting/measuring 1H-1H through-space contacts and chemical exchange, indirect TD fixed at 256 increments, default mixing time D9 = 0.6 sec (but can be modified). Gives very good signal levels on samples less than 10 mg.ĢD 1H-13C HMBC (Heteronuclear Multiple Bond Correlation), indirect TD fixed at 256 increments, filters eliminate one-bond correlations. When properly phased, CH and CH3 13C’s are positive, CH2’s are negative.ĢD COSY (COrrelation SpetroscopY), Indirect TD fixed at 200 increments.ĢD 1H-13C HSQC (Heteronuclear Single Quantum Coherence), indirect TD fixed at 128 increments multiplicity-edited so CH and CH3 peaks are positive and CH2 are negative (as in a DEPT135 spectrum), sensitivity improved over standard. ![]() ![]() Only 13C atoms with 1H’s attached are observed. 13C-detected, but signal enhanced via 1H magnetization. Change NS, but not D1, AQ, or P1, for increased signal.ġD 13C DEPT135. ![]() For all multi-scan 1H 1D spectra with more scans, use these parameters and change NS.ġD 13C, 128 scans. Parameter SetġD 1H, single scan with 90° excitation, 17 sec delay to allow for full relaxation.ġD 1H, 8 scans, optimized for maximum signal and reliable integrals. Experiment times do not include overhead for sample changing, tuning, locking, shimming, or setting gain.
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